Gfp trap chromotek
WebAlpaca GFP-Trap® is the top selling GFP antibody as it allows for one-step pulldowns that can be directly used in downstream applicaitons such as enzyme activity assays or mass … WebJul 7, 2024 · GFP-trap_A: Chromotek: Cat#gta-20: Glutathione Sepharose 4b: GE Healthcare: Cat#17075601: Dynabeads MyOne Streptavidin C1: Invitrogen: Cat#65001: Lipofectamine2000: Invitrogen: ... and supernatants incubated with affinity gel (Flag- or HA-) or GFP-trap for 90 min at 4°C. Subsequently, immunoprecipitates were washed 4x in …
Gfp trap chromotek
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WebGFP-Traps utilize super-high affinity alpaca antibody fragments coupled to agarose beads or magnetic agarose beads. These “Nanobody-Traps” are perfect for immuno-precipitation, immuno-purification, and immuno-pulldown experiments with up to 10-fold better purity (and yield) than that of conventional mouse monoclonal antibodies. http://alpaca.chromotek.com/
WebJun 23, 2024 · Split GFP technologies to structurally characterize and quantify functional biomolecular interactions of FTD-related proteins. Sci Rep. 2024;7:14013 pubmed publisher WebChromoTek GmbH, Affinity purification, Immunoprecipitation, Co-IP, Mass spectrometry, Ligand capture, Protein-protein interaction, GFP-Trap, Fluorescent protein tags, Myc-tag, Spot Capture...
WebApr 10, 2024 · Cell lysates were subjected to GFP-trap. The WCLs and GFP-trap products were immunoblotted for the indicated proteins and phospho-proteins. c, d, HEK293T cells expressing Ctrl or ILF3... WebFor immunoprecipitation of GFP-tagged proteins, the sample was first preincubated with Bab agarose beads (ChromoTek) at 4 °C for 1 h and subsequently incubated with GFP-Trap_A (ChromoTek, Planegg, Germany) at 4 °C for 3 h. The immunoprecipitated beads were washed five times with HEPES buffer containing 0.1% Triton X-100, eluted with SDS ...
WebChromoBlog Featured publications: Posted by Larisa Yurlova on May 12, 2012 1:55:00 AM High Throughput histone-tail binding assay by Pichler et al., (PLoS ONE) using our GFP-multiTrap Very nice work with GFP-Booster and RFP Booster published by Ries et al., (Nat Methods) Read More
WebHi, Iam using the GFP Trap MA from chromotek for Ip of my protein. I followed everything as per the protocol, but was not successful. My protein was seen in the flowthrough, … breathe lyrics tech n9neWebThe ChromoTek GFP-Trap™ Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. £198.00 - £4914.00 See promotional offers below cotstechWebJun 23, 2024 · Split GFP technologies to structurally characterize and quantify functional biomolecular interactions of FTD-related proteins. Sci Rep. 2024;7:14013 pubmed publisher Shwab E, Juvvadi P, Waitt G, Soderblom E, Moseley M, Nicely N, et al . cots tarragonaWebThe ChromoTek GFP-Trap® Agarose consists of an anti-Green Fluorescent Protein (GFP) Nanobody (VHH), which is covalently bound to agarose beads. GFP-Trap Agarose is … breathe lyrics michelle branchWebMay 12, 2024 · The GFP-Trap® specifically binds to most of the common GFP derivatives: The ChromoTek GFP-Trap® only binds properly folded active GFP. It is believed that this is because that nanobody binds to a three-dimensional epitope of GFP. The nanobody’s elongated CDR3 (complementarity determining region 3) allows to reach into clefts and cotstaff thorntonWebGFP-Trap® by Chromotek GmbH. Manufacturer Chromotek GmbH. 5.0. /. 5.0. 2 reviews. Write your own review. GFP -Trap® - use your … cot statement of understandingWebJun 25, 2024 · Myc-tag is a peptide tag derived from the c-Myc protein. The Myc-tag can be used for many capture and detection applications such as immunoprecipitation, immunofluorescence and protein purification. Read More Topics: Myc-tag , Spot-Tag , HA-Tag , Flag-Tag All posts breathe magazine articles