Cyto-id® green detection reagent
WebThe LYSO-ID ® Green detection kit contains an acidic organelle-selective dye suitable for live-cell staining. Conventional fluorescent stains for acidic organelles, such as Acridine Orange (Prod. No. ENZ-52405), form … Web1. Product and Company Identification Product Code: 10-4410-xx Product Name: UniCap Phosphoramidite Product Use: For Research Use Only Manufacturer Information Company Name: Glen Research LLC 22825 Davis Drive Sterling, VA 20164 USA Phone Number: 1-703-437-6191 Web site address: www.glenresearch.com Email address: …
Cyto-id® green detection reagent
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Webwere stained with Cyto-ID® Autophagy Green Detection Reagent and Hoechst 33342 nuclear stain (to account for the toxicity of screened compounds and to normalize the green signal). The accumulation of autophagic vesicles was detected using a BioTek Synergy™ Mx multiplate fluorescence reader. Cyto-ID® Autophagy Green fluorescence WebCyto-ID labeling and Flow cytometry: HEK-293 cells were harvested in Accutase solution (Merck Millipore, SF006), rinsed in DPBS and incubated for 30 min at room temperature …
WebMay 4, 2024 · The pattern of vacuole labeling 4 h after ER photodamage in the presence vs. absence of 5 μM cycloheximide with ERTracker Green, a fluorescent probe for the ER, is … WebCyto-ID Green autophagy dye staining procedure for autophagy detection Cyto-ID Green solution was prepared by mixing the dye with 1× assay buffer. The cell sample was centrifuged, and the dye was pipetted in for image-based or flow cytometry, respectively. The sample was shielded from direct light and incubated for 30 min at 37°C,
WebApr 1, 2024 · 1 INTRODUCTION. Mitophagy is a specialized form of autophagy that reduces oxidative damage by removal of damaged mitochondria and misfolded protein aggregates. 1 Amyloid-β peptide (Aβ) is found in the mitochondria of the brain tissue of AD patients and mitochondrial Aβ accumulation has been assigned a central role in the pathogenesis of … WebDec 1, 2012 · The Cyto-ID green reagent (Cyto-ID) is a. ... Lyso-ID Red Detection Reagent (Lyso-ID) were from Enzo. Life Sciences. Cell culture. Bovine aortic endothelial cells (BAEC) were obtained from.
WebProduct overview. Autophagy Assay Kit ab139484 measures autophagic vacuoles and monitors autophagic flux in live cells using a dye that selectively labels autophagic …
WebThe DYNEX Technologies Automated ELISA System is a cutting edge computer-controlled processing system that achieves full automation of ELISA assays and surrounding … solibacter是什么属WebIncase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time. ... grow the cells on coverslips after respective treatment incubate them with the dual detection reagent (Cyto‐ID dye and Hoechst 3342 nuclear stain) at 37 ºC for 30 minutes in the ... solia thermal ionic dryerWebCYTO-ID Autophagy detection kit (1x200tests) A no-transfection, quantitative assay for monitoring autophagy in live cells. CYTO-ID.The probe is a cationic amphiphilic tracer … soliath lakeWebAdvanced Security Services - Cato Networks smaiiest sized window air conditionerWebAug 27, 2024 · Prior to staining, dilute 1 μL of CYTO-ID ® Green Detection Reagent in 1 mL of phenol red free complete cell growth medium containing 5% FBS. 5. Re-suspend the pellets in 250 μL of the diluted CYTO-ID ® Green stain solution, and incubate for 30 min at 37 °C in the dark (see Note 11). 6. smail and associatesWebFeb 1, 2024 · After 24 h of laser irradiation, cells were labeled with CYTO-ID® Green. All the fluorescent images were captured using Cytation5 cell imaging multi-mode reader with objective lens 20×, followed by uniform processing using Cytation5 software (bar = 100 μm). ... To assess autophagy, we used CYTO-ID® Autophagy Detection Kit 2.0 based on a … smailagic warriorsWebApr 28, 2015 · A. parasiticus SU-1 was grown for 66 h (as described in Figure 1) in the presence of Rapamycin (500 nM, added at 24 h to induce autophagy). 6–8 colonies were stained by Enzo Cyto-ID ® Green Detection Reagent according to manufacturer’s protocol. The stained cells were observed under a fluorescent microscope as described in Methods. solibacter是什么菌