Ccf2 assay
WebAdd to cart. CCF2-FA (Free Acid) is a fluorescence resonance energy transfer (FRET) substrate that consists of a cephalosporin core linking B7-hydroxycoumarin to fluorescein. This product is intended for use as a beta-lactamase substrate in a GeneBLAzer™ cell-based assay. GeneBLAzer™ cell-based assays typically utilize the membrane-permeant ... WebOct 15, 2004 · This assay involves the use of virions containing β-lactamase-Vpr (BlaM-Vpr) and the loading of target cells with CCF2, a fluorogenic substrate of β-lactamase. Since …
Ccf2 assay
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WebSnippet: To confirm our results and to obtain further proof for the utilization of DYNA-DEP and DYNA-IND entry routes by IAV, we additionally used an IAV virus-like particle (VLP) direct entry assay [26] . These VLPs contain IAV HA and NA in their envelope and harbor a beta-lactamase reporter protein fused to the influenza matrix protein-1 (BlaM1), which … WebCCF2-FA is de-esterified and used in cell lysate applications, bypassing loading across the cell membrane and de-esterification steps. Cell lysates are the preferred method for …
Websubstrate (CCF2-AM or CCF4-AM) readily enters the cell. Cleavage by endogenous cytoplasmic esterases rapidly converts CCF2-AM or CCF4-AM into its negatively charged form, CCF2 or CCF4, which is retained in the cytosol. This substrate offers a simple and flexible assay development platform for transcriptional readouts in mammalian cells. WebCCF2-FA (Free Acid) is a fluorescence resonance energy transfer (FRET) substrate that consists of a cephalosporin core linking B7-hydroxycoumarin to fluorescein. This product is intended for use as a beta-lactamase substrate in a GeneBLAzer™ cell-based assay. GeneBLAzer™ cell-based assays typically utilize the membrane-permeant ester forms ...
WebDec 15, 2008 · CCF2 fluorescence readings were normalized by dividing fluorescence values measured at 447 nm by those measured at 520 nm, and then normalized to OD 600 of the cells. All data was then normalized to the background fluorescence measured in cells expressing cutinase only. WebFeb 18, 2024 · The CCF2 assay facilitated the analysis of injection kinetics and of intracellular accumulation levels of effectors (Mills, Baruch, Charpentier, Kobi, & Rosenshine, 2008 ). It was also successfully used …
WebAug 20, 2014 · The transfer is monitored by the enzymatic cleavage of CCF2, a fluorescent dye substrate of β-lactamase, loaded into the target cells. Cleavage of the β-lactam ring …
WebCCF2/4-AM: Greatest Asset of BLA Reporter Assay After an esterified (acetoxymethylated; AM) form of substrate CCF2/4 (CCF2/4-AM) enters a cell, endogenous esterases convert it to CCF2/4, thereby trapping it inside the cell. Exciting CCF2/4 at 408 nm leads to efficient FRET from the coumarin moiety to the fluorescein derivative and produces green … french market bistro yelpWebMar 22, 2006 · The assay could be used to specifically image endogenous cell-surface furin, urokinase plasminogen activator and metalloprotease activity. ... and CCF2/AM as the substrate because it is a ... fasting seventh day adventistWebSep 13, 2007 · The fluorometric assay is performed with a cephalosporin derivative, CCF2/AM, as the substrate . CCF2/AM contains ester moieties, which facilitate its … french market bistro menu with pricesWebDec 29, 2024 · The study of fusion of fully infectious HCV particles, HCVcc, was developed later and is based on the fusion of viral particles at the plasma membrane [ 7 ], as previously achieved for other viruses or with liposomes. Again, these assays confirmed the dependency of acid pH and for both E1E2 glycoproteins in cell entry. fasting serum glucose diabetesWebThis cleavage changes the fluorescence emission spectrum of CCF2-AM from green (520 nm) to blue (447 nm), and thus permits detection of fusion by fluorescence microscopy, … fasting serum insulin testWebJan 22, 2003 · CCF2 fluorescence on the BD LSR was collected with the use of 460/50-nm (CCF2 blue fluorescence) and 520/10-nm (CCF2 green fluorescence) bandpass filters … french market bistro lunch menuWebThe CCF2 assay facilitated the analysis of injection 95kinetics and of intracellular accumulation levels of effectors (26). It was also successfully used for HTP high content screening of T3SS inhibitors96 (27). Real-time observation of injection was achieved by direct fluorescent labeling of tetracysteine motif-tagged effectors97 (28). However, french market bistro reservations